Immune Response Induced by an Immunodominant 60 kDa Glycoprotein of the Cell Wall of Sporothrix schenckii in Two Mice Strains with Experimental Sporotrichosis

Figure 5

Presence of anti-gp60 antibodies in nonimmunized and gp60-preimmunized mice after 19 days after infection. Cell wall proteins of yeast-like cells of S. schenckii were analyzed by Western blot using sera from nonimmunized and gp60-preimmunized mice and peroxidase-conjugated secondary antibodies. Signal was revealed with a DAB-peroxidase substrate solution. Nonimmunized C57BL/6 (lanes 1–3), gp60-preimmunized C57BL/6 (lanes 4–6), nonimmunized BALB/c (lanes 7–9), and gp60-preimmunized BALB/c (lanes 10–12) mice.