Research Article

Immune Response Induced by an Immunodominant 60 kDa Glycoprotein of the Cell Wall of Sporothrix schenckii in Two Mice Strains with Experimental Sporotrichosis

Table 1

Analysis of gp60 by LC-MS/MS.

Protein nameMolecular weight (kDa)Protein scorePeptide sequence# of spectraSequence coverage (%)

Carboxy-cis, cis-muconate cyclase
S. schenckii
43.31171.6LVEMSLVNAEIIGEPIDLTTFNTDPGLTEIR
AGVSCASYSWYGLGPFDELR
TVIPGQDATCWVAICPATHTAFVTDIR
AVYVTSNTEHNSVVAIPIAR
GGNGINPR
NGSLLLNHATSTATGGR
KPVQHALLTPLGLDR
VTVVGEPAELPGEFPTTVGASDKFNLVCVGLTGAK
18542

Mass spectra were analyzed with Mascot and validated with ProteoIQ software.
Protein name: protein name as deduced by comparing peptide sequences via the software BLAST.
Molecular weight (kDa): theoretical molecular mass predicted from the amino acid sequence of the identified protein.
Protein score: sum of Mascot ion scores of all nonredundant peptides belonging to the protein.
Peptide sequence: peptides identified after LC-MS/MS analysis.
# of spectra: total number of spectra matched to the protein.
Sequence coverage: coverage of the amino acid sequence of the identified protein.