Interleukin-2/Anti-Interleukin-2 Immune Complex Attenuates Cardiac Remodeling after Myocardial Infarction through Expansion of Regulatory T Cells

Figure 1

Expansion of CD4+CD25+Foxp3+ Tregs in both spleen and mediastinal lymph nodes (mLNs) after treatment with IL-2/JES6-1 complex in vivo. PBS or the IL-2/JES6-1 complex was administered intraperitoneally in mouse for three consecutive days. CD4+CD25+Foxp3+ Treg cells from splenocytes or mLNs cells were stained and analyzed by flow cytometry on day 5. (a) Representative results of the proportion of CD4+CD25+Foxp3+ Treg cells in the spleen and mLNs analyzed by flow cytometry. (b) The percentage of CD4+CD25+Foxp3+ Treg cells within the CD4+ T cell population was enhanced in both spleen and mLNs in the IL-2/JES6-1 group. (c) The numbers of Foxp3+ T cells in spleen were also increased in the IL-2/JES6-1 group by immunohistochemical staining ( per group). Original magnification ×200. versus the PBS group.

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