Interleukin-2/Anti-Interleukin-2 Immune Complex Attenuates Cardiac Remodeling after Myocardial Infarction through Expansion of Regulatory T Cells

Figure 6

Immunofluorescent double staining of myocardial tissue with antibodies to CD68 and iNOS (a) or to CD68 and Arg-1 (b). Green indicates CD68, red iNOS or Arg-1, and blue DAPI-stained cellular nuclei. CD68+iNOS+ or CD68+Arg-1+ double positive macrophage shows yellow area (arrows). (c) The number of CD68+iNOS+ or CD68+Arg-1+ double positive macrophages in myocardial tissue from the Sham, PBS, and IL-2/JES6-1 treated mice ( per group). Original magnification ×400; versus the Sham group, versus the PBS group.

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(b)

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