Thrombin Cleavage of Osteopontin Modulates Its Activities in Human Cells In Vitro and Mouse Experimental Autoimmune Encephalomyelitis In Vivo

Figure 1

Recombinant OPN variants. (a) The figure depicts the recombinant OPN variants: OPN-FL (aa 17–314 human and aa 17–294 mouse), OPN-N including aa 17–168 (human) or 17–153 (mouse) of OPN; OPN-C including aa 169–314 (human) or 154–294 of OPN; mouse carrying a mutated thrombin cleavage site (from R₁₅₃-S₁₅₄ to S₁₅₃-F₁₅₄). (b) Western blotting showing the recombinant proteins after purification probed with the anti-His-tag (left panel) or antibodies specific for the N (middle panel) or C-terminal portion (right panel). (c) OPN-FL but not is cleaved by thrombin.

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