Thrombin Cleavage of Osteopontin Modulates Its Activities in Human Cells In Vitro and Mouse Experimental Autoimmune Encephalomyelitis In Vivo

Figure 2

Effect of OPN fragments on cytokine secretion. (a) IFN-γ, (b) IL-17A, and (c) IL-10 protein evaluated in the culture supernatants from CD4⁺ T cells by ELISA or (d) by intracellular staining with anti-IL-17A and anti-IL-10 after 5 days of treatment with OPN variants. (e) IL-6 and (f) TIMP-1 protein secreted by monocytes after 2 days of treatment with OPN variants. Data are expressed as the mean ± SE from 6 independent experiments (; versus the control; ; versus OPN-FL; ; versus OPN-C; Wilcoxon’s signed rank test).