Thrombin Cleavage of Osteopontin Modulates Its Activities in Human Cells In Vitro and Mouse Experimental Autoimmune Encephalomyelitis In Vivo

Figure 4

Effect of OPN variants on PBL migration and adhesion. (a) PBL were plated onto the apical side of matrigel-coated filters in 50 μL of medium in the presence or absence of either 10 μg/mL OPN-FL, 5 μg/mL OPN-N, or OPN-C; RANTES (10 ng/mL) was loaded in the basolateral chamber as a positive control for migration. The cells that migrated to the bottom of the filters were stained using crystal violet and counted (5 fields for each triplicate filter) using an inverted microscope. (b) PBL were pretreated or not with OPN-FL, OPN-N, or OPN-C (10 μg/mL) for 30 min, washed, and then incubated together for 1 h in the adhesion assay. Data are expressed as the mean ± SEM of the percentage of migration or adhesion versus the control obtained from untreated cells set at 100% from 5 independent experiments. (; versus the control; ; versus OPN-FL; ; versus OPN-C or OPN-N; Wilcoxon’s signed rank test).

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