A Novel System for the Quantification of the ADCC Activity of Therapeutic Antibodies

Figure 2

Expression of CD20 on the surface of Raji cells. (a, b, c) Cell surface expression of CD20 was visualized using an inverted fluorescent microscope and a FITC-labelled anti-CD20 monoclonal antibody. (d) Cell surface expression of CD20 was quantified using flow cytometry and a FITC-labelled anti-CD20 monoclonal antibody.

(a) Wild-type Raji cells

(b) CD20⁻ Raji cells

(c) CD20⁺ Raji cells

(d) Quantification of CD20 expression on wild-type and CD20 Raji cells