Decitabine-Induced Changes in Human Myelodysplastic Syndrome Cell Line SKM-1 Are Mediated by FOXO3A Activation

Figure 2

DAC induces cell cycle arrest in SKM-1 cells. (a, b) DAC treatment could decrease cells in S phase and arrest SKM-1 cells in G0/G1 and G2/M phases. (c) CDKN1A and CDKN1B levels examined in Western blot were both increased after DAC treatment in SKM-1 cells. The histogram summarizes the target/GAPDH ratio. (d) Cell cycle had clearly no change comparing FOXO3A siRNA with control siRNA. (e) Western blot showed that CDKN1A and CDKN1B levels in SKM-1 cells were decreased, but MYC was increased after FOXO3A silencing. (f) DAC-induced reduction of cells in the S phase was significantly attenuated after silencing FOXO3A. (g) DAC could not increase CDKN1A or CDKN1B protein expression in the presence of FOXO3A silencing. Student’s t-test and .

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