Decitabine-Induced Changes in Human Myelodysplastic Syndrome Cell Line SKM-1 Are Mediated by FOXO3A Activation

Figure 3

DAC induces apoptosis in SKM-1 cells. (a) Hoechst 33342 assay detected that DAC treatment could induce SKM-1 cell apoptosis. (b, c) Annexin-V-FITC/PI assay showed that apoptosis occurred in SKM-1 cells after DAC treatment. (d) BCL2L11 and FASLG proteins were examined in Western blot. BCL2L11, not FASLG, was increased after DAC treatment in SKM-1 cells. The histogram summarizes the target/GAPDH ratio. (e) Cell activity had clearly no change when compared FOXO3A siRNA with control siRNA. (f) Western blot found that BCL2L11 in SKM-1 cells were decreased after FOXO3A silencing. (g, h) FOXO3A silencing decreased the accumulation of late apoptosis of SKM-1 cells that was observed following DAC treatment. (i) BCL2L11 expression was inhibited after FOXO3A silencing and could not be induced by subsequent DAC treatment. Student’s t-test and .

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