Synergistic Antitumour Properties of viscumTT in Alveolar Rhabdomyosarcoma

Figure 3

Caspase inhibitors effectively reduce viscum- and viscumTT-mediated apoptosis. RMS-13 cells were pretreated with 50–100 μmol of caspase inhibitors Z-VAD-FMK (pan inhibitor), Z-IETD-FMK (caspase-8), Z-LEHD-FMK (caspase-9), or Z-AEVD-FMK (caspase-10) for one hour. Subsequently, cells were treated with viscum, TT, and viscumTT for 24 h and analysed by annexin/PI assay and flow cytometry. Results are presented as percentage of untreated control cells and means ± SD of three independent experiments. Mistletoe lectin (ML) and oleanolic acid (OA) concentrations were used as marker substances for viscum and TT, respectively.