Protective Effects of Moringa oleifera on HBV Genotypes C and H Transiently Transfected Huh7 Cells

Figure 4

IL-6 levels after Moringa oleifera treatment determined in the supernatant of Huh7 cells transfected with genotypes C and H. A mixture from the triplicates from the cell culture supernatant of transfected Huh7 cells was prepared with proportional volumes. ELISA was performed with a capture-specific mouse anti-human IL-6 antibody (100 μL at 2 μg/mL). After incubation and washing steps, detection was performed with Biotinylated Goat anti-human IL-6 detection antibody (100 μL at 50 ng/mL) using streptavidin-HRP.