Glycomacropeptide Attenuates Inflammation, Pruritus, and Th2 Response Associated with Atopic Dermatitis Induced by 2,4-Dinitrochlorobenzene in Rat

Figure 2

Characteristics of dermatitis-like reaction in rats challenged with DNCB after systemic sensitization. Histopathological features of the ears of control and DNCB challenged rats, 24 h after the sixth challenge, stained with (a) hematoxylin and eosin and (b) toluidine blue. Arrows indicated (a) eosinophils and (b) mast cells. (c) Eosinophils and mast cells were counted in dermis with a microscope at a magnification of 400x. (d) Ear thickness was measured at 0, 1, 6, and 24 h after last DNCB challenge. (e) To measure ear edema, equal areas from ears were punched and weighed 24 h after last challenge. (f) Scratching frequency was measured during the first 10 min after DNCB application and reported at days 16, 22, and 36. (g) Inflammatory cytokine mRNA expression in the skin lesion 24 h after the last DNCB challenge. Values represent mean ± SEM; = 5 rats. versus control at each time point.

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