Glycomacropeptide Attenuates Inflammation, Pruritus, and Th2 Response Associated with Atopic Dermatitis Induced by 2,4-Dinitrochlorobenzene in Rat

Figure 7

Effect of GMP on mRNA expression for IL-4, IL-5, IL-13, and IL-10 in ear tissue. Changes in (a) IL-4, (b) IL-5, (c) IL-13, and (d) IL-10 mRNA expression relative to β-actin were measured. Skin tissue was obtained at day 37 from control and DNCB sensitized rats, administered or not with GMP. Three rats from each experimental group were analyzed. Skin samples from each rat were analyzed in triplicate for qPCR. Each value represents the mean ± SE. Control, not sensitized and water administered before AD-induction; DNCB-P, DNCB sensitized and water administered before AD-induction; GMP-P, DNCB sensitized and GMP administered before AD-induction; DNCB-T, DNCB sensitized and water administered after AD-induction; and GMP-T, DNCB sensitized and GMP administered after AD-induction; , , and versus control; , , and versus the respective DNCB without GMP administration.

(a)

(b)

(c)

(d)