Beta-Defensin-2 and Beta-Defensin-3 Reduce Intestinal Damage Caused by Salmonella typhimurium Modulating the Expression of Cytokines and Enhancing the Probiotic Activity of Enterococcus faecium

Table 1

Primer sequences and amplification programs.

Gene	Primer sequence	Conditions	Product size (bp)
IL-6	5-ATGAACTCCTTCTCCACAAGCGC-3; 5-GAAGAGCCCTCAGGCTGGACTG-3	5at 95°C, 13 at 56°C, and 25at 72°C for 40 cycles	628
IL-8	5-ATGACTTCCAAGCTGGCCGTG-3; 5-TGAATTCTCAGCCCTCTTCAAAAACTTCTC-3	5at 94°C, 6 at 55°C, and 12at 72°C for 40 cycles	297
IL-1β	5-GCATCCAGCTACGAATCTCC-3; 5-CCACATTCAGCACAGGACTC-3	5at 95°C, 14 at 58°C, and 28at 72°C for 40 cycles	708
TGF-β	5-CCGACTACTACGCCAAGGAGGTCAC-3; 5-AGGCCGGTTCATGCCATGAATGGTG-3	5at 94°C, 9 at 60°C, and 18at 72°C for 40 cycles	439
IL-1α	5-CATGTCAAATTTCACTGCTTCATCC-3; 5-GTCTCTGAATCAGAAATCCTTCTATC-3	5at 95°C, 8at 55°C, and 17at 72°C for 45 cycles	421
TNF-α	5-CAGAGGGAAGAGTTCCCCAG-3; 5-CCTTGGTCTGGTAGGAGACG-3	5at 95°C, 6 at 57°C, and 13at 72°C for 40 cycles	324