CD80 Expressed by CD8⁺ T Cells Contributes to PD-L1-Induced Apoptosis of Activated CD8⁺ T Cells

Figure 4

Decreased Bim protein levels in activated CD80-KO CD8⁺ T cells cultured with PD-L1 as compared to WT cells. (a) ConA-activated WT and CD80-KO CD8⁺ cells were cultured with anti-CD3 (clone 2C11) and either recombinant mouse PD-L1/Fc or control/Fc (ctrl/Fc) for 48 hours, then harvested for analysis. Cells were lysed and analyzed by Western blotting for Bim and actin protein levels. Representative of two separate experiments. (b) Bim signals were quantified using ImageJ and normalized to actin signals (, ±SD, not significant).

(a)

(b)