Dysregulation of miR-155-5p and miR-200-3p and the Anti-Non-Bilayer Phospholipid Arrangement Antibodies Favor the Development of Lupus in Three Novel Murine Lupus Models

Figure 1

Detection of nonbilayer phospholipid arrangements on liposomes and antibodies on mice sera. Liposomes made of egg-yolk phosphatidylcholine (PC)/egg-yolk phosphatidic acid (PA) (2 : 1 molar ratio) in TS buffer (control) or incubated at 37°C for 30 min with the inductors of nonbilayer phospholipid arrangements: chlorpromazine 3 mM (CPZ), promazine 8 mM (PZ), or manganese 5 mM (Mn²⁺). Changes in bilayer complexity (SSC) are shown as dot plots and histograms: red lines are liposomes alone and blue lines are liposomes incubated with the inductors. Dashed lines indicate reference for changes in bilayer complexity. Kolmogorov-Smirnov test was applied; a statistically significant difference was defined by a value ≥ 0.5. One representative experiment of five is shown. Mice (four per group) were injected with PC/PA liposomes in TS buffer as negative control (SL, smooth liposomes) or liposomes incubated with chlorpromazine, promazine, or Mn²⁺. Anti-non-bilayer phospholipid arrangements, anti-cardiolipin, anti-histone, and lupus anti-coagulant antibodies were measured in mice sera before the injection of liposomes and every 15 days during 60 days after the first injection of liposomes. ND: not detected. Kruskal-Wallis and Dunn tests were applied. ; . Asterisks indicate statistical significance between the antibody titers from the three murine lupus-like models at the indicated time points.