Dysregulation of miR-155-5p and miR-200-3p and the Anti-Non-Bilayer Phospholipid Arrangement Antibodies Favor the Development of Lupus in Three Novel Murine Lupus Models

Figure 2

miRNAs obtained by PCR array analysis from the spleens of the three murine lupus-like models. (a) Heat map that indicates down- or upregulation with degrees of red and blue color, respectively. Clustergram at R Studio software was used to compare relative miRNA expression levels in the spleen of mice (four per group) injected with egg-yolk phosphatidylcholine (PC)/egg-yolk phosphatidic acid (PA) (2 : 1 molar ratio) liposomes in TS buffer (control) or liposomes incubated with the inductors of nonbilayer phospholipid arrangements chlorpromazine (CPZ), promazine (PZ), or Mn²⁺. miRNA expression was evaluated with miScript miRNA PCR array mouse immunopathology kit, each row representing an individual miRNA and each column either a murine lupus-like model or control mice. (b) Venn representation of the deregulated miRNAs. The black arrows indicate upregulation or downregulation (inverted arrows) of the corresponding miRNA.