Toll-Like Receptor Ligand-Induced Liver Injury in D-Galactosamine-Sensitized Mice: Differences between TLR7/8 and TLR9 Ligands, Cytokine Patterns, and Cross-Tolerance Induction by TLR2 Ligand Pretreatment
Table 1
Serum ALT levels in each TLRL/D-galN-treated mouse. Mean ± SD is shown.
Treatment (per mouse)
Serum ALT levels (U/1)1
C57BL/6J
C3H
PBS
5
79.8 ± 11.4
45.8 ± 12.3
D-galN 20 mg
5
71.0 ± 24.6
66.4 ± 25.2
LPS 1 μg
4
100.0 ± 16.4
96.3 ± 48.1
LPS 0.01 μg/D-galN 20 mg
5
280.3 ± 163.5
114.3 ± 38.8
LPS 0.1 μg/D-galN 20 mg
3
4830.0 ± 1429.0 (died at 5-6 h)
14713.3 ± 3131.5
LPS 1 μg/D-galN 20 mg
3
3193.3 ± 1885.0 (died at 5-6 h)
13335.0 ± 5877.2 (died at 6–9 h)
LTA 100 μg
4
83.8 ± 55.1
54.0 ± 36.8
LTA 50 μg/D-galN 20 mg
5
544.2 ± 162.1
91.8 ± 51.8
LTA 100 μg/D-galN 20 mg
5
2780.0 ± 1128.6
126.8 ± 52.6
R848 10 μg
4
90.3 ± 37.6
51.8 ± 4.4
R848 1 μg/D-galN 20 mg
5
165.4 ± 64.9
1301.2 ± 1031.7
R848 10 μg/D-galN 20 mg
5
9307.0 ± 5112.5 (died at 6–9 h)
6622.0 ± 2851.1 (died at 7–9 h)
poly (I:C) 5 μg
4
77.4 ± 20.5
63.0 ± 27.2
poly (I:C) 2.5 μg/D-galN 20 mg
5
15005.0 ± 1653.1
307.6 ± 254.7
poly (I:C) 5 μg/D-galN 20 mg
5
6486.0 ± 5779.0 (died at 5–10 h)
270.6 ± 249.5
CpG DNA 10 μg
4
76.5 ± 24.4
41.7 ± 17.4
CpG DNA 10 μg/D-galN 20 mg
5
90.4 ± 22.9
70.8 ± 16.9
CpG DNA 20 μg/D-galN 20 mg
5
10021.7 ± 3927.3 (died at 9-10 h)
318.8 ± 163.6
1The reference range (mean ± SD) of ALT has been reported to be 27.7 ± 3.09 and 25.6 ± 12.0 for C57BL/6 and C3H, respectively [20].
