Regulation of Leukocyte Recruitment to the Spleen and Peritoneal Cavity during Pristane-Induced Inflammation

Figure 1

Leukocyte responses to pristane treatment were dose dependent. Whole splenic cells were analyzed using flow cytometry after staining with antibodies specific for B220, TCRβ, CD11c, CD11b, and Gr-1 (a). The number of B cells (B220⁺), T cells (TCRβ⁺), dendritic cells (CD11c⁺), macrophages (CD11b⁺Gr1^low), or neutrophils (CD11b⁺/Gr1^hi) in the spleen was quantified (b). The difference between 100 μL of pristane treatment and 300 μL of pristane treatment in C57BL/6J mice is shown. (c) Peritoneal mesentery was collected from C57BL/6 mice treated with 300 μL of pristane and analyzed for the expression of cytokines. The difference between 300 μL pristane-treated and nontreated C57BL/6J mice is shown. Data represent two independent experiments. and .

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