Leaf-Encapsulated Vaccines: Agroinfiltration and Transient Expression of the Antigen Staphylococcal Endotoxin B in Radish Leaves

Figure 3

Cellular distributions of transient expression and encapsulation of GUS and SEB-GUS in radish leaves. (a) The majority of GUS-positive cells (blue) were located in the wounded area (arrows) of radish leaves infiltrated with A. tumefaciens (5 × 10⁷ CFU) carrying a 35S::GUS construct, but not with nontransformed A. tumefaciens (control, 5 × 10⁷ CFU). Bar = 0.5 mm. (b) Amplification of wounded areas indicated that GUS or SEB-GUS was detectable in the epidermal (arrowheads) cells, but predominantly expressed in guard cells (arrows) of infiltrated leaves. Bar = 10 μm.

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