Proteomic Identification of Heat Shock-Induced Danger Signals in a Melanoma Cell Lysate Used in Dendritic Cell-Based Cancer Immunotherapy

Figure 4

Overexpressed proteins by HS in the lysate TRIMEL. (a) Protein-protein interaction network of HS-overexpressed proteins from the lysate TRIMEL. Considering a cutoff or abs (log2 (FC)) > 1, 18 proteins were significantly more abundant in TRIMEL lysate compared to no-HS-treated lysates; however, only 17 were considered for STRING analysis. Red arrows indicate the top one overexpressed protein HP and U2SURP a previously described DAMP protein. (b) Protein-protein interaction network of the 32 proteins exclusively identified in TRIMEL samples. Red arrows indicate the exclusively identified DAMPs (HIST2H2AC, HIST2H2AA3, RRP8, and FLNC) in TRIMEL samples. Line color indicates the type of interaction evidence. Known interactions: cyan, from curated databases and pink, experimentally determined. Predicted interactions: green, gene neighborhood. Others: yellow, textmining; black, coexpression. Interaction confidence score, 0.4 (medium).

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