Luteolin Induces Apoptosis and Autophagy in Mouse Macrophage ANA-1 Cells via the Bcl-2 Pathway

Figure 3

Luteolin induces the ANA-1 macrophages apoptosis using TUNEL assay and Annexin V-FITC/PI staining. (a) The images of TUNEL-positive cells were captured by FCM. (b) The flow cytometry data show one representative Annexin V-FITC/PI staining result. (c) The ANA-1 macrophages were exposed to different concentrations of luteolin for 48 h. The values are given as mean ± SD. and versus the control. (b) The flow cytometry data show one representative TUNEL assay result. (d-e) The ANA-1 macrophages were exposed to different concentrations of luteolin for 24 h and 48 h. The rates of early- and late-stage apoptotic cell death were detected by staining cells with Annexin V-FITC and PI and analyzing by flow cytometry. The values are given as mean ± SD. and versus the control.

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