Immature Dendritic Cell Therapy Confers Durable Immune Modulation in an Antigen-Dependent and Antigen-Independent Manner in Nonobese Diabetic Mice

Figure 5

IL-2 is a contributing cytokine for the proliferating cell populations. Spleen cells prepared from different groups shown in the figure were cultured in serum-free HL-1 media for 86 hours in the presence of isotype control Ab, or neutralizing Ab against IL-2, IL-7, or IL-15. 3H-thymidine was added to culture for the last 16 hours. Data shown is representative of 10+ experiments collected through a range of posttreatment time points (13–41 weeks of age).