Expression of PD-1 on CD4⁺ Tumor-Infiltrating Lymphocytes in Tumor Microenvironment Associated with Pathological Characteristics of Breast Cancer

Figure 3

Expression of E-Cad, Ki-67, CK20, and counts of phenotypes of CD4/PD-1 TILs. (a) Patients with negative E-Cad expression (IHC, ×400). (b) Patients with positive E-Cad expression (IHC, ×400). (c) Patients with negative Ki-67 expression (IHC, ×400). (d) Patients with positive Ki-67 expression (IHC, ×400). (e) Patients with negative CK20 expression (IHC, ×400). (f) Patients with positive CK20 expression (IHC, ×400). →CD4⁺/PD-1⁺ TILs; →CD4⁺/PD-1⁻ TILs. CD4⁺/PD1⁺ TILs showed red cytomembrane and brown cytoplasm, and CD4⁺/PD1⁻ TILs showed red cytomembrane. Patients with negative E-Cad had less CD4⁺/PD-1⁻ TILs than those with positive E-Cad; patients with negative Ki-67 had less CD4⁺/PD-1⁺ TILs than those with positive Ki-67; patients with negative CK20 expression had less CD4⁺/PD-1⁻ TILs than those with positive CK20 expression.

(a)

(b)

(c)

(d)

(e)

(f)