PPAR-γ Agonist Alleviates Liver and Spleen Pathology via Inducing Treg Cells during Schistosoma japonicum Infection

Figure 3

The expression of Foxp3 and Treg cells after pioglitazone or (and) SEA stimulation in vitro. (a, b) Splenocytes from WT mice were prepared and stimulated by pioglitazone (PIO, 10 μg/ml) and a PPAR-γ antagonist (GW9662, 2 ng/ml) with or without SEA (25 μg/ml) stimulation for 24 h, and then the mRNA level of PPAR-γ and Foxp3 were analyzed by RT-PCR. (c, d) The percentage of Treg was detected after pioglitazone treatment in spleen cells with or without SEA stimulation for 24 h. All experiments were repeated three times with similar results (ANOVA/LSD). and .

(a)

(b)

(c)

(d)