Alda-1 Ameliorates Liver Ischemia-Reperfusion Injury by Activating Aldehyde Dehydrogenase 2 and Enhancing Autophagy in Mice

Figure 7

AMPK activation is involved in autophagy enhancement by Alda-1 pretreatment during liver IR. (a) Western blot analysis of p-AMPK and AMPK expression in liver tissues at 6 hours after reperfusion or sham operation (β-actin is used as a loading control). (b–d) Mice were treated with CC 1 h after Alda-1 or pretreated with DMSO and killed at 6 h after reperfusion. (b, c) Representative H&E-stained images and relative Suzuki’s scores of the liver section. PT: portal triads; CV: central veins. Scale bars: 50 μm. Scale bars: 50 μm. (d) Serum ALT/AST level. (e) Western blot analysis of p-AMPK, AMPK, Bcl2 and Bax, LC3B, and P62 expression in liver tissues (β-actin is used as a loading control). All data are shown as mean ± SEM (n = 4–6). .

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