Construction and Characterization of Adenovirus Vectors Encoding Aspartate-β-Hydroxylase to Preliminary Application in Immunotherapy of Hepatocellular Carcinoma

Figure 2

Agarose gel electrophoresis of gradient PCR products. The total AAH mRNA was extracted from surgically resected HCC tissues; the PCR products were amplified with the use of commercially obtained primers and validated by 0.8% agarose gel electrophoresis. Lane 1: 500 bp marker; lane 11: 100 bp marker; lanes 3–9: gradient AAH PCR products, respectively, representing annealing temperature: 56°C, 58°C, 60°C, 62°C, 64°C, 66°C, 68°C; lane 2: negative control group; lane 10: positive control group, 462 bp.