Research Article
Construction and Characterization of Adenovirus Vectors Encoding Aspartate-β-Hydroxylase to Preliminary Application in Immunotherapy of Hepatocellular Carcinoma
Figure 4
Expression of the AAH gene in dendritic cell (DC) vaccines. (a) The fluorescent (①) and visible (②) images of DCs infected by Ad-AAH-IRES2-EGFP at a MOI of 200 after 36 h infection (100x). (b) Expression of AAH protein in DC vaccines by western blot. (c) The expression percentage of typical phenotypes of mature DCs by FCM analysis. Compared to the GFP-DC group or blank DC group, the maturation markers of DCs in the AAH-DC group were significantly highly expressed; , . (d) The typical phenotypes of DCs by FCM.
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