PM2.5 Exposure in the Respiratory System Induces Distinct Inflammatory Signaling in the Lung and the Liver of Mice

Figure 1

Two-photon intravital imaging of neutrophils in the lung and the liver of mice exposed to particulate matter 2.5. (a) Images show the difference between PM2.5 before and after sonication. Scale bar, 10 μm. LysM-GFP mice were treated with PM2.5, and the number of neutrophils was recorded 24 h and 48 h after treatment via two-photon intravital imaging of mouse lung (b) and liver (c), respectively. See Videos 1, 2, 3, and 4. The blood vessel was stained with Dextran-Texas red. Scale bar, 50 μm. The number of neutrophils at 10 min was counted from two-photon imaging of mouse lung (d) and liver (e), respectively. There was no significant increase in the neutrophil number in the lung and liver tissues compared to that in the control group.

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