Preliminary Evaluation of the Safety and Immunogenicity of an Antimalarial Vaccine Candidate Modified Peptide (IMPIPS) Mixture in a Murine Model

Figure 7

Immunofluorescence assay with mouse anti-IMPIPS antibodies in Plasmodium falciparum-infected erythrocytes (FCB2 strain). Results were analysed semiquantitatively according to fluorescence intensity from null (0+) to maximum fluorescence (+++). (a) PBS (negative control) (0+). (b) Serum from a P. berghei-infected mouse (positive control) (+++). (c) Serum from the group of mice immunised with IMPIPS plus adjuvant (++). (d) Serum from the group immunised with IMPIPS plus PSS (+). Each study was done in duplicate. Mouse serum and the FITC-labelled anti-mouse IgG (green fluorescence) were used at 1 : 20 dilution. The samples were analysed with a fluorescence microscope (Olympus B51) with an immersion objective (1,000x).

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