Efficient Acquisition of Fully Human Antibody Genes against Self-Proteins by Sorting Single B Cells Stimulated with Vaccines Based on Nitrated T Helper Cell Epitopes

Figure 2

NitraTh contributes to antigen-specific IgM⁺ B cells formation in vitro. (a) Detection of HER2-specific IgM in culture supernatants of DC-T-B cell coculture system by ELISA assay. and compared with HER2; ^### compared with HER2-Th. (b) HER2-specific IgM-secreting B cells were detected by ELISpot assay. A total of HER2-specific B cells were present in every B cell of the coculture system. compared with HER2-Th. (c) Detection of CB1-specific IgM in culture supernatants of DC-T-B cell coculture system by ELISA assay. and compared with CB1; ^## compared with CB1-Th. (d) CBR1-specific IgM-secreting B cells were detected by ELISpot assay. A total of CB1-specific B cells were present in every B cell of the coculture system. compared with CB1-Th.

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