Efficient Acquisition of Fully Human Antibody Genes against Self-Proteins by Sorting Single B Cells Stimulated with Vaccines Based on Nitrated T Helper Cell Epitopes

Table 1

HER2-NitraTh can activate CD4⁺ T cells with different HLA class II haplotypes.

Sample	Genotype		Proliferation rate of naïve CD4^+ T cells (%)
	HLA-DR	HLA-DQ	Blank	HER2-NitraTh
1	DRB101; DRB109	DQB103; DQB105		()
2	DRB111; DRB115	DQB103; DQB106		()
3	DRB107; DRB114	DQB102; DQB105		()
4	DRB108; DRB115	DQB103; DQB106		()
5	DRB107; DRB115	DQB102; DQB106		()
6	DRB104; DRB109	DQB103; DQB103		(n.s.)
7	DRB101; DRB112	DQB103; DQB105		()

Naïve CD4⁺ T cells were sorted from peripheral blood and stimulated with HER2-NitraTh for 7 days, and the proliferation rate was measured by FACS. Meanwhile, the HLA-DR and HLA-DQ loci of donor PBMCs were detected by PCR amplification with sequence-specific primers.