Lipopolysaccharide-Induced Transcriptional Changes in LBP-Deficient Rat and Its Possible Implications for Liver Dysregulation during Sepsis

Table 1

Associated genes enriched in representative GO terms and pathways. (a) Representative GO terms and related genes involved in the WT and LBP-deficient groups after LPS injection. (b) Interested pathways enriched from DEGs between WT and LBP-deficient rats.

(a)

Category		GO	Terms	Associated genes	P-value
WT	0 h	GO:0006656	Phosphatidylcholine biosynthetic process	APOA2, FABP5	3.5E-02
		GO:0008289	Lipid binding	APOA2, PACSIN3, FABP5	4.2E-02
	6 h	GO:0051607	Defense response to virus	IFIT3, IFIT2, IFNAR2, OASL, ZC3HAV1, IFNG, CXCL9, CXADR, CXCL10	5.9E-06
		GO:0010942	Positive regulation of cell death	CDKN1A, GZMBL2, ZC3H12A, GZMB, FAS, BCL2L11	5.5E-05
		GO:0032496	Response to lipopolysaccharide	SELP, TNFRSF9, JUN, SOCS1, SERPINE1, IL1RN, CXCL9, LBP, FAS, PCK1, CXCL10	5.5E-05
		GO:0006954	Inflammatory response	SELP, NFKBIZ, TNFRSF9, OLR1, C4B, CXCL9, ZC3H12A, FAS, CELA1, CXCL10	4.4E-04
		GO:0007159	Leukocyte cell-cell adhesion	SELP, OLR1, TNIP1, NT5E	8.7E-04
		GO:0006955	Immune response	RT1-A2, TNFRSF9, GZMBL2, CXCL9, GZMB, FAS, SECTM1B, RT1-BB, CXCL10	9.9E-04
		GO:0071222	Cellular response to lipopolysaccharide	SERPINE1, IFNG, ZC3H12A, LBP, ABCA1, CXCL10, ADAM9	1.7E-03
		GO:0042157	Lipoprotein metabolic process	OLR1, APOL9A, ABCA1	1.8E-02
		GO:0006925	Inflammatory cell apoptotic process	IFNG, FAS	2.3E-02
	24 h	GO:0006953	Acute-phase response	HNRNPK, IL1RN, ITIH4, LBP, LOC100911545	1.9E-04
		GO:0006641	Triglyceride metabolic process	APOE, APOA5, CYP2E1, SLC22A5	3.4E-03
		GO:0006635	Fatty acid beta-oxidation	ECI1, EHHADH, DECR1, CROT	5.1E-03
		GO:0006629	Lipid metabolic process	SLC16A1, HNF4A, APOE, IL1RN	3.0E-02
		GO:0034380	High-density lipoprotein particle assembly	APOE, APOA5	5.9E-02
		GO:0006869	Lipid transport	APOE, APOA5, LBP	7.6E-02
LBP^-/-	0 h	GO:0055114	Oxidation-reduction process	FMO5, CYP4A2, D2HGDH, HSD17B2, GPX4, CYP7A1, CYP2C7, DPYD, CYP8B1, POR	2.8E-03
		GO:0005504	Fatty acid binding	ACOX2, CYP4A2, ADH4	7.8E-03
		GO:0005739	Mitochondrion	ACOX2, D2HGDH, TRMT1L, SDS, GPX4, ADH4, MRPS10, VDAC2, PTEN, GPT2, ACSF2, POR, LRP5	4.9E-02
	6 h	GO:0055114	Oxidation-reduction process	CYP4A2, PYCR2, PIR, CYP4F6, FASN, ADH6, NQO1, DHRS7B, DDO, CYP2A3, FDFT1	1.2E-03
		GO:0031998	Regulation of fatty acid beta-oxidation	TYSND1, CPT1A	2.4E-02
		GO:0008611	Ether lipid biosynthetic process	FASN, DHRS7B	2.9E-02
		GO:0001666	Response to hypoxia	CD38, RAMP2, HSP90B1, CLDN3, ANGPTL4	4.2E-02
		GO:0043666	Regulation of phosphoprotein phosphatase activity	HSP90B1, RCAN1	4.7E-02
		GO:0006629	Lipid metabolic process	APOE, ACLY, MID1IP1	6.9E-02
	24 h	GO:0006006	Glucose metabolic process	LOC108351137, ONECUT1, MYC, FABP5	7.5E-03
		GO:1904385	Cellular response to angiotensin	CYBA, MYC	5.9E-02
		GO:0035457	Cellular response to interferon-alpha	MNDA, MYC	7.6E-02
		GO:0030097	Hemopoiesis	SGPL1, CIAPIN1, CSF1R	8.3E-02
		GO:0005739	Mitochondrion	NADK2, ABCF2, NUDT6, MRPS12, MRPS10, PLGRKT, QARS, LOC100359687, COMT, GLYATL1, FIS1,; CYBA, SCCPDH, CHCHD10, ACSL1, KRAS, PARL, P2RY2, TIMM9, HEBP1, PXMP2, APEX1, MYC	4.5E-04
		GO:0005811	Lipid particle	SCCPDH, PNPLA3, ANXA2	6.5E-02

(b)

Category		ID	Pathways	Associated genes	P-value
WT	0 h	/	/	/	/
	6 h	rno05320	Autoimmune thyroid disease	RT1-A2, RT1-CE7, GZMBL2, GZMB, FAS, RT1-BB	8.0E-04
		rno04066	HIF-1 signaling pathway	EGFR, CDKN1A, PFKFB3, HMOX1, SERPINE1, IFNG	2.8E-03
		rno04060	Cytokine-cytokine receptor interaction	IFNAR2, TNFRSF9, IFNG, CXCL9, EDAR, FAS, CXCL10	1.2E-02
		rno04620	Toll-like receptor signaling pathway	IFNAR2, JUN, CXCL9, LBP, CXCL10	1.4E-02
		rno04650	Natural killer cell mediated cytotoxicity	IFNAR2, GZMBL2, IFNG, GZMB, FAS	1.4E-02
		rno04152	AMPK signaling pathway	IRS2, CCND1, PFKFB3, CAB39, PCK1	3.4E-02
	24 h	rno03320	PPAR signaling pathway	CYP4A1, EHHADH, APOA5, FABP1, FABP7	5.0E-03
		rno00071	Fatty acid degradation	ECI1, CYP4A1, EHHADH, ADH6	8.6E-03
		rno04060	Cytokine-cytokine receptor interaction	CCL12, TNFSF10, LIFR, IL2RG, IL1A, CSF1R, ACVR1	8.9E-03
		rno04931	Insulin resistance	SREBF1, PTPRF, GFPT1, PIK3CA	7.7E-02
LBP^-/-	0 h	rno03320	PPAR signaling pathway	ACOX2, CYP4A2, RXRA, CYP7A1, CYP8B1	7.6E-04
		rno00140	Steroid hormone biosynthesis	HSD17B2, CYP7A1, CYP2C7	7.1E-02
	6 h	rno01100	Metabolic pathways	CYP4A2, NAGS, NAT1, B3GALT4, ADH6, ACLY, FDFT1, CD38, PYCR2,; UGT1A3, CYP4F6, GMPPA, FASN, LOC100912599, PAPSS1, CYP2A3	1.6E-02
		rno00071	Fatty acid degradation	CYP4A2, ADH6, CPT1A	3.8E-02
		rno03320	PPAR signaling pathway	CYP4A2, CPT1A, ANGPTL4	9.1E-02
	24 h	rno01100	Metabolic pathways	NADK2, SGPL1, QARS, COMT, PSPH, PNPLA3, GMPS, UMPS, ACSL1,; GBE1, DPM3, LOC100912599, UGT2A3, ALG11, AMD1, FLAD1, CYP2C22	3.2E-02