Knockdown of lncRNA CCAT1 Inhibits the Progression of Colorectal Cancer via hsa-miR-4679 Mediating the Downregulation of GNG10

Figure 5

hsa-miR-4679 reversed the phenotype of GNG10 on promoting CRC. (a) WB was performed to detect the level of GNG10 after GNG10 overexpression. (b) EdU staining was performed to detect the proliferation ability after the treatment with hsa-miR-4679 mimics, GNG10 overexpression, and hsa-miR-4679 mimics+GNG10 overexpression. (c) The statistical result of (b). (d) WB was performed to detect the levels of proliferation markers. (e) Transwell migration and invasion assays were performed to access the migration and invasion abilities after the treatment with hsa-miR-4679 mimics, GNG10 overexpression, and hsa-miR-4679 mimics+GNG10 overexpression. (f) FACS was performed to analyze the apoptosis rate after the treatment with hsa-miR-4679 mimics, GNG10 overexpression, and hsa-miR-4679 mimics+GNG10 overexpression. (g) The statistical results of (e). (h) The statistical results of (f). and .

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