The Macrophage Activator GcMAF-RF Enhances the Antitumor Effect of Karanahan Technology through Induction of M2–M1 Macrophage Reprogramming

Table 1

The sequences of primers used in this study.

Primers	Oligonucleotide sequences
IL-1β-for	5’-TCCAGGATGAGGACATGAGCAC-3’
IL-1β-rev	5’-GAACGTCACACACCAGCAGGTTA-3’
TNFa-for	5’-AAGCCTGTAGCCCACGTCGTA-3’
TNFa-rev	5’-GGCACCACTAGTTGGTTGTCTTTG-3’
IL-10-for	5’-GACCAGCTGGACAACATACTGCTAA-3’
IL-10-rev	5’-GATAAGGCTTGGCAACCCAAGTAA-3’
IL-12a-for	5’-TGTCTTAGCCAGTCCCGAAACC-3’
IL-12a-rev	5’-TCTTCATGATCGATGTCTTCAGCAG-3’
TGF-b-1-for	5’-GTGTGGAGCAACATGTGGAACTCTA-3’
TGF-b-1-rev	5’-TTGGTTCAGCCACTGCCGTA-3’
NOS2-for	5’-TCACCTTCGAGGGCAGCCGA-3’
NOS2-rev	5’-TCCGTGGCAAAGCGAGCCAG-3’
Arg1-for	5’-GATTATCGGAGCGCCTTTCT-3’
Arg1-rev	5’-CCACACTGACTCTTCCATTCTT-3’
Ido1-for	5’-AGGATCCTTGAAGACCACCA-3’
Ido1-rev	5’-CCAATAGAGAGACGAGGAAG-3’
GAPDH-for	5’-AAATGGTGAAGGTCGGTGTG-3’
GAPDH-rev	5’-TGAAGGGGTCGTTGATGG-3’

for, a forward primer; rev, a reverse primer.