SerpinB3/B4 Abates Epithelial Cell-Derived CXCL8/IL-8 Expression in Chronic Rhinosinusitis with Nasal Polyps

Figure 1

SerpinB3 and B4 expression in nasal tissues of patients with CRSwNP: (a, b) SerpinB3 and SerpinB4 mRNA levels in nasal tissues of ECRSwNP and nonECRSwNP patients measured by real-time PCR. n = 24 for ECRSwNP patients, n = 16 for nonECRSwNP patients, and n = 15 for healthy controls. RNA expression is measured from ABI ViiA™ 7 real-time PCR system and calculated as arbitrary units (2^(–Δct) × 1,000). Data are presented as mean ± SD and shown through Graphpad prism 8.0. GAPDH is used as the reference gene, (c, d) SerpinB3 and B4 protein levels in nasal tissues of nonECRSwNP and ECRSwNP patients and healthy controls measured by western blotting, and (e, f) intensities of western blot bands measured by image lab. Protein levels were normalized to those of GAPDH. n = 8 for ECRSwNP and nonECRSwNP patients and healthy controls. , , . CRSwNP, chronic rhinosinusitis with nasal polyps; ECRSwNP, eosinophilic CRSwNP; SerpinB, serine proteinase inhibitor, clade B; and GAPDH, glyceraldehyde 3-phosphate dehydrogenase.

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