SerpinB3/B4 Abates Epithelial Cell-Derived CXCL8/IL-8 Expression in Chronic Rhinosinusitis with Nasal Polyps

Figure 2

SerpinB3 and B4 expression in noninvasive samples from patients with CRSwNP: (a, b) SerpinB3 and SerpinB4 mRNA levels in nasal brush cells of ECRSwNP and nonECRSwNP patients measured by real-time PCR. n = 12 for ECRSwNP patients, n = 14 for nonECRSwNP patients, and n = 16 for controls. RNA expression is measured from ABI ViiA™ 7 real-time PCR system and calculated as arbitrary units (2^(–Δct) × 1,000). Data are presented as mean ± SD and shown through Graphpad prism 8.0. GAPDH is used as the reference gene and (c, d) SerpinB3 and B4 protein levels in nasal secretions of nonECRSwNP and ECRSwNP patients measured by ELISA. Protein content were measured by BioTek Epoch 2 Microplate spectrophotometer and produced by Epoch Gen5 software. Data are presented as mean ± SD and shown through Graphpad prism 8.0. n = 16 for ECRSwNP patients, nonECRSwNP patients, and healthy controls. , . CRSwNP, chronic rhinosinusitis with nasal polyps; ECRSwNP, eosinophilic CRSwNP; SerpinB, serine proteinase inhibitor, clade B; and GAPDH, Glyceraldehyde 3-phosphate dehydrogenase.

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