SerpinB3/B4 Abates Epithelial Cell-Derived CXCL8/IL-8 Expression in Chronic Rhinosinusitis with Nasal Polyps

Figure 5

Genes and pathways in response to SerpinB3 and B4 stimulation. RNA sequencing analysis of ALI-cultured HNECs (n = 4) after 24 hr stimulation with recombinant SerpinB3 and B4, respectively: (a, b) volcano plots showing DEGs in response to SerpinB3 and B4. The red dots represent statistically upregulated DEGs while the green dots represent downregulated DEGs, (c, d) PPI networks of the 144 and 280 DEGs of SerpinB3 and B4, respectively. DEGs are arranged in circles by degree. The depth of the node color, font size, and node size reflect the degree of connection (darker colors, larger font size, and larger node size represent higher degrees). Top hub genes in the PPI networks are presented at the center of the circles, and (e, f) KEGG pathway enriched by the downregulated DEGs of SerpinB3 and B4, respectively. The number of genes enriched based on the enrichment terms is represented by the size of the node. PPI, protein–protein interaction; DEGs, differentially expressed genes; KEGG, kyoto encyclopedia of genes and genomes; SerpinB, serine proteinase inhibitor, clade B; ALI, air–liquid interface; and HNECs, primary human nasal epithelial cells.

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