SerpinB3/B4 Abates Epithelial Cell-Derived CXCL8/IL-8 Expression in Chronic Rhinosinusitis with Nasal Polyps

Figure 6

CXCL8 is downregulated by SerpinB3 and B4 in nasal epithelial cells: (a, b) PPI networks constructed by downregulated DEGs involved in the inflammatory pathway. The degree of genes is represented by the depth of the color and the size and font of the node, (c) CXCL8 expression validated by real-time PCR (n = 5), (d) the intracellular levels of IL-8 in cultured HNECs that treated with recombinant SerpinB3 and B4 (n = 4), (e) the concentration of IL-8 in supernatant of cultured HNECs that treated with recombinant SerpinB3 and B4 (n = 5), (f) expression of SerpinB3 and B4 after transfection with SerpinB3/B4 siRNA in HNECs (n = 7), detected by real-time PCR. SerpinB3/B4 siRNA was designed for simultaneously knockdown of SerpinB3 and B4. Black column represents siNC group, and orange column represent siSerpinB3/B4 group, (g) the gene expression of CXCL8 after transfected with SerpinB3/B4 siRNA in HNECs (n = 7), and (h) the production of IL-8 in supernatant of cultured HNECs (n = 7) that transfected with SerpinB3/B4 siRNA. , . PPI, protein–protein interaction; DEGs, differentially expressed genes; SerpinB, serine proteinase inhibitor, clade B; HNECs, primary human nasal epithelial cells, siNC, negative control siRNA, and siSerpinB3/B4, SerpinB3/B4 siRNA.

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