An Aloe-Based Composition Constituting Polysaccharides and Polyphenols Protected Mice against D-Galactose-Induced Immunosenescence

Figure 2

Cytotoxicity effect of UP360 and its constituents. PBMCs were added at a 25 : 1 ratio of PBMCs to K562s, and treated at concentrations of 25, 7.5, 15, and 2.5 µg/mL with UP360, aloe, Poria, and Rosemary, respectively. The plates were scanned using an automated cell imaging system at baseline, and every hour for 4 hr. The number of live cells was analyzed for each well by adjusting the detection parameters for fluorescence intensity and cell diameter. The percent cytotoxicity for each treatment was calculated as the difference in live cells at each time point subtracted from the baseline count, divided by the baseline count. The reported percent cytotoxicity beyond the normal control was calculated as the difference in percent cytotoxicity between the treatment and the normal control: (a) percent cytotoxicity beyond the normal control and (b) representative cell imaging at 0 and 4 hr.

(a)

(b)