The Interaction of Plasma Sialylated and Desialylated Lipoproteins with Collagen from the Intima and Media of Uninvolved and Atherosclerotic Human Aorta

Table 2

Binding of sialylated and desialylated IDL to collagen isolated from two layers of the intima and the media of uninvolved and atherosclerotic areas of human aorta.

Lipoprotein	Collagen-bound apoB, pg/well
	PG-rich layer	Musculoelastic layer	Media
Uninvolved area
Sialylated IDL	512 ± 21	325 ± 27	385 ± 27
Desialylated IDL	559 ± 61	393 ± 12	418 ± 19
Initial lesion
Sialylated IDL	547 ± 27	270 ± 32	237 ± 19
Desialylated IDL	554 ± 18	287 ± 19	229 ± 7
Fatty streak
Sialylated IDL	214 ± 4^&	236 ± 6	234 ± 14
Desialylated IDL	243 ± 17^&	296 ± 13	258 ± 14
Fibroatheroma
Sialylated IDL	331 ± 18^&	187 ± 16	260 ± 18
Desialylated IDL	419 ± 19^*&	238 ± 2*	276 ± 12

Each well of 96-well microtiter plates was coated with collagen and washed as described in Section 2. The binding of IDL (10 μg/mL) was performed at 37^oC for 2 h. Values are the means ± S.E.M (n = 4); *significant difference from sialylated IDL (𝑃<0.05); ^&significant difference from uninvolved region (𝑃<0.05).