The Interaction of Plasma Sialylated and Desialylated Lipoproteins with Collagen from the Intima and Media of Uninvolved and Atherosclerotic Human Aorta

Table 3

Binding of sialylated and desialylated LDL to collagen isolated from two layers of the intima and the media of uninvolved and atherosclerotic areas of human aorta.

Lipoprotein	Collagen-bound apoB, pg/well
	PG-rich layer	Musculoelastic layer	Media
Uninvolved area
Sialylated LDL	507 ± 27	331 ± 8	408 ± 13
Desialylated LDL	416 ± 47	350 ± 10	353 ± 15
Initial lesion
Sialylated LDL	363 ± 18^&	373 ± 12	426 ± 14
Desialylated LDL	374 ± 14	374 ± 7	517 ± 7
Fatty streak
Sialylated LDL	277 ± 5^&	332 ± 26	356 ± 17
Desialylated LDL	290 ± 5	317 ± 17	403 ± 12
Fibroatheroma
Sialylated LDL	460 ± 11^&	365 ± 13	458 ± 15
Desialylated LDL	539 ± 16*	508 ± 13*	527 ± 15

Each well of 96-well microtiter plates was coated with collagen and washed as described in Section 2. The binding of LDL (10 μg/mL) was performed at 37°C for 2 h. Values are the means ± S.E.M (n = 4); *significant difference from sialylated LDL (𝑃<0.05); ^&significant difference from uninvolved region (𝑃<0.05).