Impact of oxLDL on Cholesterol-Rich Membrane Rafts

Figure 1

Oxidized LDL induces translocation of caveolin from the plasma membrane to intracellular compartments. Endothelial cells were treated with 10 μg/mL of nonmodified LDL (nLDL) or oxLDL (15–20 nmol/mg TBARS) in 100% lipoprotein-defficient serum containing 20 mm HEPES (pH 7.4) for 1 h at 37°C. Caveolin was visualized with a rhodamine isothiocyanate-goat antirabbit IgG. Adapted from [21].