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Figure 3: Visualizing noncaveolae lipid rafts by proximal imaging of GPI-anchored tt-GFP. Human umbilical vein endothelial cells are transfected with GPI-anchored temperature-tolerant green fluorescent protein (ttGFP) probe and exposed either to cholesterol oxidase (chol ox, 0.5 U/l) for 60 minutes or 2 mM methyl-β-cyclodextrin (MβCD) for 30 minutes. Adapted from [23].