The gangliosides follow the same endocytic pathway as FcεRI. At 15 minutes of incubation with both mAb BC4-gold (which recognizes the α subunit of FcεRI) and mAb AA4-HRP (which recognizes gangliosides derived from GD1b), the BC4-gold (arrows) and AA4-HRP (arrowheads) are colocalized in early endosomes adjacent to the plasma membrane (PM).
