Paraoxonases-2 and -3 Are Important Defense Enzymes against Pseudomonas aeruginosa Virulence Factors due to Their Anti-Oxidative and Anti-Inflammatory Properties
Figure 1
PON2 or PON3 overexpression diminishes ROS production induced by P. aeruginosa signaling molecule pyocyanin (PCN). (a) Naïve, PON2-GFP, or PON3-GFP overexpressing HEK293 cells were loaded with carboxy-H2DCFDA and stimulated with PCN (2.4 μM). Carboxy-H2DCFDA fluorescence as means of ROS was recorded over several hours. (b) Similar to A. Naïve, PON2-GFP, PON3-GFP, or PON3-dsRed overexpressing EA.hy 926 cells were loaded with L-012 and stimulated with PCN (2.4 μM). Curve maxima calculated by nonlinear regression showed statistically significant differences () between naïve and PON2 or PON3 overexpressing cells.
