A Nonradioactive Fluorimetric SPE-Based Ceramide Kinase Assay Using NBD-C6-Ceramide
Figure 2
Influence of water and acidity on fluorescence of NBD-derivatives. Fluorescence of methanolic NBD-C6-Cer-1-P solutions (1 μM, 200 μL) containing increasing amounts of water (a) different acids at 0.5 N final concentration (b) or increasing TFA concentration (c) (λ ex 465 nm, λ em 535 nm; Tecan Infinite 200).
