Fatty Acid Composition of Phospholipids and in the Central and External Positions of Triacylglycerol in Muscle and Subcutaneous Fat of Beef Steers Fed Diets Supplemented with Oil Containing n6 and n3 Fatty Acids While Undergoing One of Three 48 h Feed Withdrawal Treatments

Table 5

Eicosapentaenoic acid (EPA) and Docosahexaenoic acid (DHA) composition of phospholipid (PL), triacylglycerol (TAG), and at sn-2 monoglycerol (sn2) and sn1/3 of fat from the pars costalis diaphragmatis (PCD) and subcutaneous fat (SQ) of beef steers fed dietary oil composed of an equal mixture of flax oil and sunflower oil at 5% of diet and in one of no feed withdrawal (No FW), single feed withdrawal (FW × 1), and feed withdrawal every 8 wk (FW × 4) for 48 h treatments.

Item	Location	Control			Oil			Probability
		No FW			No FW			Diet	FW
PCD
EPA (wt%)	PL; TAG; sn2; sn1/3							0.1187; 0.0770; 0.8375; 0.8317	0.7509; 0.5379; 0.8203; 0.8283	0.4996; 0.4211; 0.8609; 0.8195
DHA (wt%)	PL; TAG; sn2; sn1/3							0.8727; 0.2593; 0.4054; 0.4733	0.8110; 0.3560; 0.6429; 0.6922	0.4051; 0.0700; 0.1939; 0.2150
SQ
EPA (wt%)	PL; TAG; sn2; sn1/3							0.3893; 0.5538; 0.6681; 0.4934	0.8285; 0.8194; 0.5088; 0.4209	0.2728; 0.4455; 0.1804; 0.1026
DHA (wt%)	PL; TAG; sn2; sn1/3							0.7232; 0.9240; 0.5004; 0.8162	0.8102; 0.9113; 0.2041; 0.1331	0.9032; 0.4261; 0.0225; 0.0714

as in Paterson et al. [10], n  = 12.