Humanization of 4E9. (a) The g2e6p mutations are shown in green spheres; the three additional mutations M55L, A126T, and V206T, are shown in blue spheres; the 4E9 mutations (with respect to G3C9) are shown in red spheres. (b) Thermal inactivation of hum-4E9 produced with and without MBP fusion is compared to huPON1 and 4E9. Residual activity here was from EMP hydrolysis rather than phenyl acetate because the H115W mutation renders PON1 inactive against phenyl acetate.
